Isotope tracting for NO chemiluminescence assays

نویسندگان

  • Julia Cornelius
  • Tuan Tran
  • Nicole Turner
  • Abigail Piazza
  • Lauren Mills
  • Ryan Slack
  • Sean Hauser
  • J. Steven Alexander
  • Matthew B. Grisham
  • Martin Feelisch
  • Juan Rodriguez
چکیده

Biological Chemistry ’Just Accepted’ Papers are papers published online, in advance of appearing in the print journal. They have been peer-reviewed, accepted and are online published in manuscript form, but have not been copy edited, typeset, or proofread. Copy editing may lead to small differences between the Just Accepted version and the final version. There may also be differences in the quality of the graphics. When papers do appear in print, they will be removed from this feature and grouped with other papers in an issue.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

DNA Methyltransferase Activity Assays: Advances and Challenges

DNA methyltransferases (MTases), a family of enzymes that catalyse the methylation of DNA, have a profound effect on gene regulation. A large body of evidence has indicated that DNA MTase is potentially a predictive biomarker closely associated with genetic disorders and genetic diseases like cancer. Given the attention bestowed onto DNA MTases in molecular biology and medicine, highly sensitiv...

متن کامل

Chemiluminescence: sensitive detection technology for reporter gene assays.

A series of enzyme-activated chemiluminescence-based assays of reporter gene expression, useful in many biomedical applications, has been developed. The chemiluminescence detection systems for beta-galactosidase, beta-glucuronidase (GUS), and secreted placental alkaline phosphatase (SEAP) reporter enzymes are all based on use of 1,2-dioxetane substrates. This detection technology also permits t...

متن کامل

A Search for Isotope Effects in Chemiluminescent Reactions of Metastable Ca*(PJ, D2) Atoms with CH3I and CD3I Molecules

Chemiluminescent reactions of calcium atoms in the metastablePJ and D2 states with CH3I and CD3I were studied in a beam-gas arrangement. Calcium monoiodide spectra associated with transitions from the electronic A Π, B Σ and C Π states to the X Σ ground state were recorded. Total collision and chemiluminescence cross sections were measured. It was found that isotopic substitution in the methyl ...

متن کامل

Chemiluminescence microscopy as a tool in biomedical research.

Chemiluminescence has become a standard tool in biomedical research. Chemiluminescent probes are used for immunoassays, nucleic acid identification, reporter gene assays, measuring enzyme activity, and the detection of ions and small molecules such as Ca2+, ATP, NO, O2- and H2O2. Along with the development of new chemiluminescent probes, significant progress has been made in techniques to measu...

متن کامل

Comparison of three different methods in the assessment of neutrophil function.

Three different methods to measure the oxidative respiratory burst of neutrophils were performed. Of the three, the chemiluminescence technique was observed to be the most sensitive among them. The strong statistical correlation and an acceptable agreement between chemiluminescence with that of the killing assay provides evidence for using the chemiluminescence assay as an alternative method of...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2013